Mouse embryonic hepatic stem cells differentiate into cardiomyocyte-like cells

doi:10.3969/j.issn.2095-4344.2013.32.011

Abstract

Abstract:

BACKGROUND: In recent years, embryonic hepatic stem cells have attracted more attention, but there are few reports on the potential of embryonic hepatic stem cells to differentiate into cardiomyocyte-like cells as well as the related differentiation conditions.
OBJECTIVE: To investigate the moderate condition to induce mice embryonic hepatic stem cells to differentiate into cardiomyocyte-like cells in vitro with chemical reagents.
METHODS: Dimethylsulfoxide in combination with 5-azacytidine with different concentrations and time were used to induce the embryonic hepatic stem cells of 13.5 days mice and to observe the differentiation effect.
RESULTS AND CONCLUSION: Under in vitro conditions, 0.8% dimethylsulfoxide+5 μmol/L 5-azacytidine could induce the mouse embryonic hepatic stem cells to express the specific markers of myocardial cells, while increasing the concentration of the inducer and extending the induction time could not improve the induction efficacy.

Key words: stem cells, embryonic stem cells, embryonic hepatic stem cell, dimethylsulfoxide, 5-azacytidine, induction, differentiation, cardiomyocyte-like cells, α-actin, troponin T, stem cell photographs-containing paper

CLC Number:

R394.2

Wu Bi-gang, Chang Jing, Zhang Xiao-gang2 . Mouse embryonic hepatic stem cells differentiate into cardiomyocyte-like cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(32): 5813-5818.

Figures/Tables 3

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